FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_b4_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_b4_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8819572
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161380.1829794008144613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC18700.033.0246146
TATGCCG19100.032.6938948
ATGCCGT24300.026.12389249
GTATGCC24350.026.07024847
TCGTATG24400.025.73403445
TCGGGCG14500.024.5060527
CGCGCGT13150.023.6140331
CTCGTAT27250.022.28306644
GGCGGGC15250.021.71777713
CGCGTAT10500.021.687431
CGCGCCT22500.021.161671
CGGGCGC16050.020.85010515
TGCCGTC30850.020.46526150
GCGGGCG16750.019.97886814
CGGCAAT26400.019.081051
ATCGGGC18950.018.7514386
CGCGGAT12000.018.1139321
GTCACGT37400.017.06540529
GGGCGGC20050.017.0345219
GCGCCTG26000.016.9848982
Produced by FastQC (version 0.11.2)