FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_b4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_b4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11225817
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193730.17257541255126463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC23350.032.35812446
TATGCCG23600.032.30743848
CCGCACA26400.029.01079836
CGTCCGC28550.026.82633633
TCCGCAC29150.026.39204635
GTATGCC29400.026.16867347
CGCACAT29750.025.51210637
CGCGCGT14350.025.4874571
GTCCGCA31500.023.98556534
TCGTATG31700.023.94360245
ATGCCGT32650.023.66970849
ACGTCCG32900.023.17452832
CGCGTAT13400.022.402011
CTCGTAT36350.020.88085444
GCACATC38300.019.4565438
CGGGCGC15750.018.83772715
TCGGGCG16500.018.8176487
GGCGGGC15900.018.44312113
TGCCGTC43100.018.33092350
GCGGGCG15850.018.28355414
Produced by FastQC (version 0.11.2)