FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_b4_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_b4_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11466775
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG26400.030.31850446
CGTATGC27300.029.06636844
ATGCCGT31750.025.53559347
GTATGCC32500.024.41564445
TCGTATG34450.023.43380443
CGCGTAT11700.023.0016481
TGCCGTC36650.022.40386448
CGCGCGT13500.022.2349261
CGCGGAT17800.020.3525831
CTCGTAT41500.019.120142
CGCGCCT23350.017.2881761
CGGGCAT20050.017.2082161
TCACGTA46450.017.15726530
TCGGGCG13600.016.9959857
ACGTAGA48950.016.63289532
GCCGTCT50200.016.49419849
GTCACGT51050.015.74642529
AGTCACG52050.015.6427528
CACGTAG52650.015.59513131
CGGGCGC13800.015.49989315
Produced by FastQC (version 0.11.2)