FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_b3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_b3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11150086
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC19550.031.94151746
TATGCCG22350.027.78515448
TCGGGCG21200.026.5247977
CGGGCGC21550.023.69313615
GGCGGGC22150.023.05133613
TCGTATG27500.022.70751445
GTATGCC27450.022.49750547
ATGCCGT27750.022.37881749
GCGGGCG23000.022.34943414
ATCGGGC25500.022.1880726
CGCGTAT13850.022.1717991
CGCGCGT15550.021.5229631
GGGCGGC25550.020.6588769
CGGCAAT34950.019.8431031
TCCGTAT32150.019.20808437
TAGCCCG26950.018.81779134
CGTATCT33450.018.66785639
CTCGTAT33250.018.57321244
CGGGCAT23000.018.4517671
CCGTATC34250.018.33254838
Produced by FastQC (version 0.11.2)