FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_sw4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_sw4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14736788
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG158000.10721467934532274No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT41800.029.21774169
ACGCGTG71000.029.10637769
TACGCGT99800.020.6723768
GTCTAGT34800.018.1524261
TAGCCTA18150.018.05781210
GTATAAC37000.017.2596841
TTACGCG135800.016.91975669
CCCATGC69000.016.5999668
AGTTCCG29700.016.4951365
TCACACG49250.016.39156529
TAGAGCG28900.016.3548585
ACCCCCC17300.016.3525648
CCATGCC77950.015.5791849
GTATAGG17300.015.5636441
GTTAAAT64750.015.4071011
AGAGCGT30750.015.2585556
CGTGTAG24000.015.23751969
TCTCCCG25300.014.86335314
CTGATCG32550.014.6266979
CGGCCAG38400.014.4729631
Produced by FastQC (version 0.11.2)