FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_sw3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_sw3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17343573
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC86750.017.4983358
TCACACG72200.017.29746229
CCATGCC92500.016.5970849
AGTTCCG40800.015.558765
GTCTAGT49050.015.1315961
CACACGT89100.014.28761330
CAAACGG98350.013.39985315
TCCCATG116300.013.3489267
CTTAGGT92050.013.30509969
TTCACAC100500.013.21617228
CACGTTC97700.013.2065132
TAAATCC113200.013.1057493
GCTTAGG97050.012.97510468
TGTTATA63100.012.9595562
GTTATAC67100.012.9573993
GTATATA60900.012.9241751
AAATCCC107150.012.8146924
ATCCCAT128350.012.7676586
GTGTTAT99050.012.6862191
GTTAAAT125500.012.6806791
Produced by FastQC (version 0.11.2)