FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_sw2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_sw2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16761572
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC67750.015.8368268
CCATGCC72050.015.4183869
TCACACG51750.015.13308129
GTCTAGT44600.013.54505351
TAGCCTA21500.013.47900610
ATACTCC196550.013.14697569
GATACTC243050.012.2498969
CGCCCGG11350.012.1585336
GTATTAA46450.011.96513751
GTATAGA44200.011.9494171
GTATATA54150.011.7937191
GTATAAG42550.011.7637551
TACTCCT177750.011.74256169
GCCCGGG13000.011.6768687
CGGCCAG28400.011.6689141
CCTTGTG108050.011.46269469
ATCCCAT101750.011.4264816
CACACGT67950.011.42365130
GTTAAAT98650.011.4076991
AGTTCCG37700.011.34752755
Produced by FastQC (version 0.11.2)