FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_sw1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_sw1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14171769
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGTG81150.017.04786769
AGTGTGT64800.016.07852269
GCAGTGT109750.013.73697569
GTATAGA47650.012.6781541
GTATATA80450.011.843061
GTATTAG41450.011.8261851
GATATGC289500.011.72629469
TATGCAG201600.011.29451669
CGATATG330400.011.05783569
TGCAGTG146100.011.05120969
ATATGCA262200.010.89461369
GTATAAG43850.010.8640161
GTATAGG32750.010.6461111
GTATTAA50350.010.3528131
GGACCGT18100.09.911496
GTACTAG30150.09.8467291
TTATACT62650.09.7471384
ATGCAGT174550.09.74408969
TATACTG68100.09.6763425
GTATTAT81500.09.5302831
Produced by FastQC (version 0.11.2)