FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_sw0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_sw0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10871573
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG144030.13248312824648284No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCCC16300.019.26040818
TTACGTG42100.018.0282869
CCCATGC44650.017.7712548
CCGCGAG5300.017.5751727
TACGTGT29400.017.48447669
TGCACGC17500.017.15113616
CCGACGC16050.016.98109869
CGGCCAG20200.016.7481231
CCATGCC48650.016.239199
TCACACG36650.016.0964429
ACGCCCT20900.015.18634519
CTTACGT56300.014.584187569
GCCAGAC23500.014.2414293
GACACGG23950.014.1166717
GTATATA34300.013.7885281
CGCCCTT23850.013.59725320
GCACGCC23950.012.9642917
CACGGAT23800.012.9010429
TAGCCTA17250.012.79978310
TTATACT34300.012.6737794
Produced by FastQC (version 0.11.2)