FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_sq4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_sq4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12367911
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC44050.014.5674638
GGAGCGC16950.014.247622
CCATGCC48600.013.8425229
GCGCGTG18750.013.6158226
TCACACG35450.013.13799729
CCTAAGC15000.013.1107283
CGCGTGA19250.012.72450827
GTATATA53050.012.6237871
TAGCCTA17350.012.32846710
ATGCGCG20200.012.29686724
TGCGCGT21200.012.20502825
GAGCGCT21000.011.66413223
AGTTCCG26000.011.2791545
GTATAAA67000.011.1289011
GTCTAGT34200.011.1029881
GTATTAG45500.011.0768121
GTGTAAG36300.011.0312471
CAAACGG50100.010.94891315
GTATTAA46600.010.8894191
GTATAGG25200.010.8218091
Produced by FastQC (version 0.11.2)