FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_sq3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_sq3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16133254
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC102900.021.8262448
TCACACG90600.020.75297529
CCATGCC108900.020.4652989
CACACGT99300.018.9694830
CAAACGG109100.018.4355415
CATGCCA129300.017.20975110
TTCACAC113050.017.08955828
TCCCATG133050.017.0099247
TAAATCC129550.016.8318083
ATCCCAT137650.016.6921166
AGTTCCG42200.016.6778165
AAATCCC125350.016.5413764
ACACGTT116150.016.4848831
CACGTTC118900.016.27770232
AATCCCA132250.016.2261775
GTTAAAT140600.015.8613681
CCAAACG128950.015.81168614
CGCCCGG12150.015.617086
GTCTAGT47300.015.3997881
GCCCGGG13050.015.3331337
Produced by FastQC (version 0.11.2)