FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_sq2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_sq2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11845199
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC62250.018.84328
TCACACG54300.017.85327529
CCATGCC67100.017.584049
CACACGT61450.015.77596230
CAAACGG71600.015.12962115
TCCCATG83750.014.870977
GTTCCGA37900.014.1171741
ATCCCAT92900.014.1118776
CACGTTC73150.013.81862132
AAATCCC80000.013.7568054
ACACGTT72150.013.62761931
TAAATCC86600.013.4660863
AGTTCCG29900.013.3846045
AATCCCA88400.013.3082485
CCAAACG82450.013.30601114
GTCTAGT36100.013.2911611
TTCACAC75100.013.27606328
CATGCCA91150.012.83092910
CGCGTGA26500.012.75831927
GTATATA47800.012.709841
Produced by FastQC (version 0.11.2)