FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_sq1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_sq1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17679650
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC76150.018.257888
TCACACG61300.018.0094729
CCATGCC85200.016.6019619
CACACGT77050.014.50720830
GTATAGG31500.013.1502131
GTATATA54750.013.0511361
GTCTAGT54550.013.03570651
GTATAAA76900.012.8381311
CACGTTC90600.012.68020532
GCGCGTG32650.012.67975226
CAAACGG94100.012.57519615
CGCGTGA31150.012.51506427
CCGCGAG8050.012.4284597
TTCACAC92550.012.37583128
TCCCATG111950.012.3268197
TAAATCC107200.012.2944273
ATCCCAT121700.012.2463966
ACACGTT95850.012.09365131
TGCGCGT34800.011.99551325
AGTTCCG40400.011.955545
Produced by FastQC (version 0.11.2)