FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_sq0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_sq0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22476152
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACACG94050.021.6423229
CCCATGC121250.021.1977128
CCATGCC127450.020.7891089
CACACGT107350.019.25020630
TAAATCC134300.017.8033473
GTTAAAT145500.017.415211
GTATATA34000.017.3625261
TAGCCTA31250.016.78062210
AAATCCC138400.016.4522464
CGAGGGA58450.016.4087257
GTATAGG40000.016.225331
TTCACAC128700.016.2177428
CACGCCC32950.016.12422218
GTATTAG41150.016.1074621
CACGTTC135850.015.74499932
ATCCCAT176400.015.6852066
TTAAATC146150.015.628842
CAAACGG139150.015.59473615
TCCCATG166900.015.5858137
GTCTAGT71400.015.5687351
Produced by FastQC (version 0.11.2)