FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_other_stuff_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_other_stuff_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17208578
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC72150.012.8155833
GTATTAG65900.012.4158231
CGATCTA113100.012.35405869
GTATAGA78700.012.2827591
GTATAGG66000.012.0831621
TATACAG91600.012.014865
CACGCAG47050.011.87866316
CGGTCGG15750.011.828382513
GTCGGGG16300.011.64091915
TATATAC149350.011.0423783
ACTATGC80300.010.9557448
TCGGGGG17500.010.84268416
TACAGGC48100.010.8305147
GTATATA134600.010.7725081
GATCTAC109850.010.67815169
TAATACT129650.010.5376174
TATACCG43350.010.5052975
ATACAGG73100.010.3357966
GTATAAA114200.010.3086281
GTCCTAA38200.010.2123971
Produced by FastQC (version 0.11.2)