FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_other_stuff_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_other_stuff_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12512441
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG58500.016.5825161
TCCGCAC24300.016.46892564
CCGCACC24550.016.44174665
CGCACCT26500.015.49225566
CTAATAC64450.014.8818273
TAGGTAG28350.014.7248585
GTATAGA62800.014.5675411
GTATATA145650.014.5294781
TATACAG83600.013.7422145
TCTATAC58550.013.3762183
TATACCG38500.013.3519385
TAATACT107600.013.3382264
TATTAGG59450.012.8840642
TATACGG44800.012.8604825
TATACTG85100.012.5675585
CTTACAC30200.012.1096983
GTGTAAG29950.011.9877191
GTATTAT109800.011.9161751
CATATAC86050.011.9080313
GTATAAG55100.011.9042481
Produced by FastQC (version 0.11.2)