FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_hw4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_hw4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15308110
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG194050.1267628727517636No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC17950.018.83551869
TACGTGT39000.016.98449169
CCGCGAG9450.016.0633227
TTACGTG54000.015.97210669
CCCATGC54000.015.5887248
CGATCCG8650.015.155910510
TCACACG45150.014.89998129
CCATGCC58400.014.5323829
AGTTCCG26100.014.0114855
GACGCGC30050.013.77689438
CTCGCCG14750.013.56578228
TCGTTCG32250.013.4788421
GGTCTCG32050.013.45526517
CGTTCGT33050.013.04818822
CTAGTAG51550.012.984353
CCTGCCG44000.012.93723716
GCCTCGT35950.012.85926519
GTCTAGT35600.012.7997771
TGACGCG32450.012.75795537
CTTAAGG42250.012.6576223
Produced by FastQC (version 0.11.2)