FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_hw3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_hw3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10854834
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG161150.1484592026004267No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCCG26750.021.4093885
CCCATGC65700.020.3742838
TCACACG57900.019.06713329
CCATGCC71000.018.9991599
GTCTAGT32900.017.3120351
AAATCCC74200.016.8314934
CACACGT68250.016.42837730
CAAACGG75200.016.33225415
ACACGTT72050.015.75345831
CATGCCA87300.015.72840910
AATCCCA82600.015.7046315
ATCCCAT88700.015.480166
CTCTGTG87750.015.45117169
TCCCATG89650.015.3930857
CTCGCCG11100.015.22954928
GTTAAAT90450.015.1510261
ATCGTAC32000.014.9874872
TCTGTGT73500.014.87948569
TAAATCC88600.014.7972773
CCAAACG84400.014.79721814
Produced by FastQC (version 0.11.2)