FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_hw2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_hw2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17627512
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG191670.10873343895610452No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA91500.017.72113669
ATAGTGT184800.017.30583469
AGTGTAG103400.016.31560369
GATAGTG244650.015.92075169
GGATAGT305200.014.3899369
TAGTGTA131850.012.89975868
AGGATAG370200.012.82321269
GAGGATA475800.011.59414769
AGAGGAT580750.011.45335169
TAGACCG30350.011.140085
TATACCG38550.010.828845
AAGAGGA776000.010.4966169
GTATAAG53800.010.1374771
GAAGAGG902900.010.12942769
CGAAGAG946500.010.10021769
GTATAGA55150.010.0770971
ACCTCGC20250.09.7110428
TATACGG41650.09.6915185
GTATTAG50500.09.6379091
GGACCGT21150.09.6240456
Produced by FastQC (version 0.11.2)