FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_hw1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_hw1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17687751
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG178690.10102471478708629No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC75450.019.5246148
TCACACG64850.018.40676529
CCATGCC82300.018.1929749
CCGCGAG11450.017.7770777
GAGGTGT137950.016.88100669
CACACGT75150.015.97576330
AGTTCCG35400.015.7883465
CCCGGGG12150.015.3441221
GTCTAGT46900.015.1641791
CTAGCCG14050.014.7339993
ATCCCAT109650.014.7248556
GTGTAGA66050.014.57295669
TCCCATG103950.014.5698087
CAAACGG92000.014.36224415
ACACGTT87650.014.09102131
GAGAGGT248900.014.01336869
AAATCCC97300.013.89942554
CGGCCAG28250.013.5652891
CACGCCC27400.013.47238818
AGAGAGG289700.013.27830469
Produced by FastQC (version 0.11.2)