FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_hw0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_hw0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15222800
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTG8850.017.15261369
CCCATGC45450.015.4851118
CCATGCC49700.014.7162629
TCACACG33450.014.1297829
GTATTAG37950.014.0081571
GTATAAA63350.012.9688771
GTATTAA50250.012.5028021
GTATAGA41650.011.5205131
GTATATA46800.011.50671
TCTATAC39700.010.8633373
CGCGTGT8950.010.79334269
CACACGT44500.010.77620130
GTATAAG43200.010.70762351
CGCGTGA13250.010.67527127
TATAAAG72100.010.4326572
TCCCATG72100.010.3356217
GTATAGG42150.010.2372781
TATAAGG45200.010.2291612
CAGCCCG11500.010.19980823
GTATTAT88900.010.1346971
Produced by FastQC (version 0.11.2)