FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_hq4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_hq4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13283056
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC46850.017.0105348
TCACACG39500.016.68195329
CGCTAGG9100.015.92300746
CCATGCC51200.015.767459
CGTACTG34100.015.1760594
CACACGT47550.014.22054430
TACGGGA35950.014.10697839
ATACGGG37300.014.058866538
GATCGTA37000.013.7148541
AGTTCCG23550.013.3313045
TGCGTAA26200.012.77279810
GTCTAGT32550.012.6203541
GACGGGA16850.012.48941828
GGACGGG16300.012.48758227
TTGCGTA26400.012.4146719
ATCGTAC40050.012.3192732
TCCCATG67450.012.2245587
CGCGTAG11050.012.1762820
CCTACCG43500.012.13438411
GCCTAGT11300.011.90702867
Produced by FastQC (version 0.11.2)