FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_hq3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_hq3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16794766
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGC22050.017.99293538
GTATATA21000.017.9195791
CGCGCAT22650.017.51630240
GTATAGA35600.017.261981
TGACGCG25000.016.5597637
CGCTAGG10450.016.17679846
GTATTAA32100.016.025141
CCCATGC60950.015.9621758
GTATTAG30250.015.6356451
CGGCTCG8400.015.60691564
CCCGCGT8400.015.60691547
TAGCCTA17100.015.53510
ACGCGCA26500.015.23185539
TATACAG36650.015.1557115
GTATAAC39850.015.0744531
TCACACG45550.014.996429
TATAGAG28800.014.9760152
TATATAA20450.014.1730952
TATATAC21100.014.0622023
CACACGT50350.014.04638530
Produced by FastQC (version 0.11.2)