FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_hq2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_hq2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13829894
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAG10050.025.7459987
CGCTAGG11250.020.85311546
GACTCCC15250.019.6817643
CCCGGGG6150.019.6455881
CGCGAGG12850.019.5989468
CTAGCCG14750.018.2453943
CTCCCGT18550.017.85426145
CTCGGCC13400.017.76467527
AGTTCCG27000.017.2499455
ACTCCCG18800.017.0663144
CCCATGC62550.016.8774598
CCTCGGC14300.016.64661826
CCATGCC66100.016.5973459
TCACACG52750.016.35048129
CCCGTGG20800.015.59118247
GCCGCGA17450.015.0256296
GCATATC50100.015.0207251
GTCTAGT36150.014.9921341
GCCTAGT14700.014.78555967
CACACGT59300.014.77719530
Produced by FastQC (version 0.11.2)