FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_hq1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_hq1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16053360
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAG12600.027.9283337
CTAGCCG15900.022.1329213
CTCGGCC15500.021.81258227
CGCGAGG16600.021.406388
CACGCCC29900.020.5381618
CGCACCG16950.020.15027251
GCCGCGA17650.020.1329756
TGCACGC31500.019.16638416
GCACGCC33100.018.55259717
CGGCCAG33700.018.5401421
CCCATGC72400.018.0598748
TCACACG59250.018.05036729
AGTTCCG33950.017.885315
AGCCGCG20300.017.8450095
ACGCCCT35100.017.49546819
CGCTAGG11850.017.46820646
CGGTGCG18300.017.344069
CCATGCC77200.017.205119
CCTCGGC19700.017.16218426
CACGGAT36000.017.1539699
Produced by FastQC (version 0.11.2)