FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_hq0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_hq0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15229971
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAG8100.024.2778477
CTAGCCG11150.018.8750533
CACGCCC20850.018.36659818
GACTCCC14300.016.64668343
TGCACGC23450.016.33021716
CGGCCAG27800.016.1429981
CCCATGC57500.015.9000468
CCCGGGG7350.015.4993051
CGCGAGG12700.015.4842978
CGCACCG12950.015.45157951
TCACACG47150.015.36559529
GCACGCC25600.015.09349517
ACGCCCT26000.014.99397919
CCATGCC63350.014.9219069
CGGTGCG13700.014.6058829
CGATCCG7750.014.24520210
CACGGAT28100.014.1192589
CTCCCGT18050.013.95276545
GCCAGAC29650.013.8469933
GGACGGG15200.013.3913127
Produced by FastQC (version 0.11.2)