FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_ew4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_ew4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14827585
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC91300.019.9894858
CCATGCC94700.019.2353769
TCACACG80200.019.05643729
AGTTCCG46150.018.7640295
GTCTAGT56900.017.4721951
CAAACGG99150.015.97095415
AAATCCC105100.015.88784
CACACGT99150.015.48387330
CACGTTC100650.015.42444732
CCCGGGG10400.015.2683381
ATCCCAT127050.015.206536
GTTCCGA54350.015.1710316
TCCCATG124300.015.1543827
TAGTTCC62250.015.0193494
TTCACAC103500.014.99976728
ACACGTT107200.014.7394631
TAAATCC118400.014.599353
CGTACTG52200.014.4742064
AATCCCA127550.014.3898155
CCAAACG112000.014.20017814
Produced by FastQC (version 0.11.2)