FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_ew3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_ew3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14190294
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC89400.018.0987728
TCACACG77850.017.77043329
AGTTCCG42350.017.5148535
CCATGCC93750.017.3325889
GTCTAGT52500.016.7673951
CAAACGG97400.015.08908515
AAATCCC101350.014.97788054
CACACGT92850.014.9367630
CCAAACG106350.014.37071914
CACGTTC100000.014.35177432
GTTCCGA50500.014.3463596
ACACGTT102350.014.22449431
TCCCATG116250.014.1262797
CAGTGTG65350.014.09551669
ATCCCAT119550.014.053786
TAAATCC114350.013.969573
AATCCCA113800.013.6727445
TTCACAC105350.013.52475228
TAGTTCC59800.013.1539164
ACGTTCT109850.012.93925933
Produced by FastQC (version 0.11.2)