FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_ew2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_ew2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15495393
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC102350.019.246978
TCACACG89050.018.8284129
CCATGCC106200.018.3867939
GTCTAGT55050.017.8716131
AGTTCCG49950.017.3364725
TTACGTG67150.017.00581669
TACGTGT50250.016.752169
CAAACGG109300.016.12917115
TCCCATG126600.015.66925057
CACACGT106150.015.60028530
CACGTTC112850.015.49949832
AAATCCC118600.015.4174394
ATCCCAT131200.015.2776436
CCAAACG118300.015.10623814
ACACGTT117050.014.70755331
AATCCCA132950.014.2983455
CTTACGT92100.014.19695169
TTCACAC120650.014.18296528
TAAATCC133500.014.1369323
GTTAAAT143850.014.0145521
Produced by FastQC (version 0.11.2)