FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_ew1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_ew1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11480675
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC67000.018.9490768
CCATGCC70750.018.2372849
TCACACG57800.018.02570229
GTCTAGT44050.015.594381
AGTTCCG36900.015.5203145
CAAACGG73550.015.24452215
CACACGT71350.014.84422530
CGTACTG39750.014.8414994
ATCCCAT90650.014.7284886
TCCCATG88800.014.6856737
CCCGGGG8250.014.6445171
CACGTTC75650.014.59332332
CCAAACG79100.014.52382114
AAATCCC79700.014.3280954
ACACGTT77950.014.16272931
ATCGTAC43300.013.7859762
GTTCCGA41850.013.6844596
TAGTTCC49550.013.5771864
AATCCCA93400.013.4823255
CGATCTA124750.013.44035369
Produced by FastQC (version 0.11.2)