FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_ew0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_ew0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11223735
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC53200.017.6387658
CCATGCC58200.016.65699
TCACACG48600.016.18480729
CAAACGG62450.013.20305415
CACACGT60350.013.14799430
TCCCATG75200.012.983197
ATCCCAT80450.012.8649476
CACGTTC65800.012.79301532
AAATCCC70300.012.7106834
ACACGTT64450.012.63275431
GCGCGTG21200.012.36774626
CGCGTGA20450.012.31522627
GTATATA58350.012.2464261
CCAAACG70800.012.03573614
ATCGTAC38450.012.0246792
AGTTCCG29900.012.0001485
TAAATCC83250.011.8945293
TGCGCGT23500.011.89131725
GATCGTA39500.011.8856151
GGAGCGC14250.011.86303822
Produced by FastQC (version 0.11.2)