FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_eq4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_eq4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12432472
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC60050.018.6143118
CCATGCC64750.018.168949
TCACACG51750.017.99975829
TAGCCTA24650.017.21484810
CACGTTC65700.014.80801932
GTATATA48450.014.6764421
CAAACGG67950.014.571547515
TGCGCGT25700.014.49785925
GTCTAGT46350.014.2987751
GCGCGTG25150.014.2662126
CGCGTGA24200.014.25600727
CACACGT65600.014.25209530
ATGCGCG25250.014.07307724
GCGACTG28950.014.06198716
CTCCTAA35100.013.5712331
AGTTCCG34400.013.5393345
GTATTAG30500.013.4677321
TCCTAAC36300.013.4021562
TTCACAC70900.013.33268328
TCCCATG83050.013.2930727
Produced by FastQC (version 0.11.2)