FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_eq3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_eq3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16219556
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC84500.018.8218298
CCATGCC89750.018.297439
TCACACG72250.018.1926829
CAAACGG92600.015.57309215
TCCCATG109700.015.5045047
CACACGT86550.015.06726230
ATCCCAT113700.014.8983666
AAATCCC97850.014.7379054
GTATAGA47800.014.2276061
CACGTTC97200.014.01975332
GTATATA60300.013.9690221
CCAAACG104650.013.94474914
AATCCCA114700.013.8663395
TTCACAC96950.013.80685628
TAAATCC114200.013.686213
GTATAGG37750.013.6258251
GTTAAAT119600.013.566261
GTCTAGT57000.013.4453351
ACACGTT103800.013.36097831
GTATTAG39950.013.2211171
Produced by FastQC (version 0.11.2)