FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_eq2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_eq2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16489010
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC98900.020.8951598
CCATGCC102450.020.4068439
TCACACG87150.020.10985229
AGTTCCG42950.016.9488815
ATCCCAT129450.016.8967196
AAATCCC112200.016.850324
CACACGT106750.016.44986730
CAAACGG111100.016.3647415
TCCCATG128000.016.1986837
AATCCCA125500.016.109265
TTCACAC115550.015.55536828
CACGTTC117700.015.44709132
GTCTAGT57650.015.3897271
TAAATCC127350.015.3071713
CCAAACG121150.015.29196914
GTTAAAT135000.014.8828561
ACACGTT122350.014.86001331
TAGCCTA31950.014.57733310
GTATTAG39100.014.4798541
CATGCCA141600.014.20432210
Produced by FastQC (version 0.11.2)