FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_eq1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_eq1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15045599
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC84450.020.017618
ACGCGTG31350.019.80846469
TCACACG72500.019.36724729
CCATGCC88350.019.2901769
TACGCGT46800.017.47102469
AGTTCCG40400.016.9084595
GTCTAGT51300.016.623031
CGCGTGT25500.016.37046669
CAAACGG93250.015.83457715
CACACGT91250.015.50109930
ATCCCAT112950.015.3943076
AAATCCC96750.015.3332694
TTACGCG59650.015.09544469
CGCGTGA27900.014.96210427
TCCCATG114500.014.9147357
GTATTAG38500.014.7066481
CACGTTC98500.014.53527932
CCAAACG103100.014.52253914
ACACGTT100250.014.24713231
TAAATCC109750.014.2098253
Produced by FastQC (version 0.11.2)