FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n02_eq0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n02_eq0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14356719
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC87950.019.1425728
AGTTCCG44100.018.7756525
TCACACG78150.018.7616829
CCATGCC92300.018.7263159
GTCTAGT57150.016.9135591
CAAACGG95050.016.11545815
ACGGCCC23150.016.09492910
AAATCCC100100.015.8541554
ACTCCCG14200.015.79204244
GACTCCC14000.015.77121743
ATCGTAC54100.015.5620882
CCCGGGG9800.015.4995761
GATCGTA57050.015.3699071
GTTCCGA53100.015.2682646
CACACGT97350.015.09681730
CACGTTC99550.015.07508532
ATCCCAT118000.014.9693966
TCCCATG117050.014.9140437
CCAAACG103450.014.90695114
CGTACTG59400.014.8106144
Produced by FastQC (version 0.11.2)