FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_sw4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_sw4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14736788
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG16250.019.95678369
TGAGCAT100950.018.04444969
TCACACG47200.017.98178129
GTCTAGT34350.017.978591
TAGCCTA17250.017.60005610
CCCATGC70250.017.0909678
GCCAGAC40250.016.972463
CCATGCC76150.016.9444739
GTTAAAT65000.016.3480781
ACCCCCC18400.016.3130518
TATGCCG22650.016.29767843
TCTCCCG26800.016.09130514
CGGCCAG41000.016.0723291
GTATTAT41450.016.064311
TAAATCC68250.015.9745973
AGTTCCG28350.015.8211755
GTATAAC37650.015.669691
CCCGCCT27650.015.59663517
GTATAGA22450.015.52152251
CACACGT55050.015.47961230
Produced by FastQC (version 0.11.2)