FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_sw3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_sw3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17343573
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC90600.018.430678
CCATGCC94050.018.1214149
TCACACG74500.017.45903829
CGCGTGA32600.015.13386727
CACACGT90850.014.46876730
CAAACGG100400.014.46671215
GCGCGTG36200.014.39133626
AGTTCCG42950.014.3790465
AAATCCC108650.014.194354
ATCCCAT124350.014.1503086
GTCTAGT51450.014.1489691
TCCCATG121750.014.0270537
TAAATCC115300.013.9143013
TGCGCGT38000.013.70964125
ATGCGCG37550.013.41449624
CACGTTC103900.013.31554932
GTTAAAT124800.013.2694941
TTCACAC101750.013.15614928
CATGCCA130050.013.13152110
CCAAACG114000.013.10390614
Produced by FastQC (version 0.11.2)