FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_sw2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_sw2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16761572
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC72400.016.3927028
TCACACG51550.015.25992629
CCATGCC79500.015.2323029
GTCTAGT47450.014.0328731
GTATTAG39050.013.6058351
CGGCCAG32050.013.4557371
GTATAGG35450.012.8464531
AGTTCCG37350.012.7476725
GCCAGAC38250.012.7181763
GACAGGC233650.012.66887269
CAGGCAG160150.012.58062969
ACAGGCA204800.012.4825969
CACGCCC35700.012.27299718
TGCACGC35450.012.26219416
TCCCATG100650.012.1002477
CCGCGAG12550.012.0960137
CACACGT66850.011.97335830
GTATATA59800.011.9424851
GACACGG42550.011.9193097
ATCCCAT109650.011.7366086
Produced by FastQC (version 0.11.2)