FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_sw1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_sw1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14171769
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGGC273350.012.5328469
GTATAGA52200.012.2271471
GTATTAG44850.011.8462851
CAGGCAG192550.011.66424869
ACAGGCA245500.011.31263369
GTATATA77800.010.9975511
GTATAGG29900.010.6155011
AGACAGG345600.010.5017469
TAGACAG35300.010.4582135
CTAATAC47100.010.3286133
TAATACT66000.010.2983484
ATAGGAC24650.010.0776523
GTATAAA69750.010.0409711
TATACTG65900.09.947565
TATAATA78900.09.9263512
GTCTAAG33100.09.9019221
TATTATA80850.09.9003082
GTATAAG41050.09.7491941
GTATATG61100.09.7120551
TATACCG33100.09.6940085
Produced by FastQC (version 0.11.2)