FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_sw0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_sw0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10871573
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG17050.022.25869869
CGTATGC24100.019.611641
TATGCCG23700.019.50589843
TGCACGC16900.018.98504816
CCGCGAG6550.018.9628097
CACGCCC17050.018.81793818
CCCATGC48650.018.722538
CCATGCC49750.018.3775779
TCACACG39850.017.74863629
GTATGCC28400.016.8851942
CGGCCAG20700.016.4998631
ATGCCGT28250.016.4863644
CGCACCG10750.016.04615851
TCTCGTA29350.015.63338538
GACACGG22750.015.62058457
ACGCCCT20600.015.57504119
CCCGGGG6900.014.9998751
CACGGAT21400.014.9935569
ATCTCGT30800.014.56136737
CACACGT49550.014.5519830
Produced by FastQC (version 0.11.2)