FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_sq4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_sq4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12367911
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGGG3800.018.1580221
CCTAAGC15200.015.2077753
CCCATGC44950.015.1204868
TCACACG34550.015.07874229
CCATGCC49950.014.2282729
CGCGTGA19150.013.8726127
GCGCGTG20200.013.32230926
ATGCGCG21800.012.18640824
CACACGT43650.012.17210530
TGCGCGT22200.012.122148525
TATACTG43550.012.1211275
TCCTAAG22800.011.8030012
GTATAGA36550.011.7989881
GTATTAG42850.011.6745281
GTATATA47900.011.3080141
CCGAGCT21550.011.20625957
TAGCCTA17950.011.14762110
GTATAGG28450.011.0352271
GTATTAA50800.011.0020471
GTATATG53900.010.9453471
Produced by FastQC (version 0.11.2)