FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_sq3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_sq3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16133254
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC103650.021.6027818
CCATGCC109500.020.8264339
TCACACG92900.020.05452229
CACACGT101350.018.48415430
CCCGGGG14350.017.7910121
CAAACGG114650.017.78419715
TCCCATG130200.017.4095947
CATGCCA129350.017.36342410
ATCCCAT135700.016.7550836
AATCCCA129450.016.7111035
AAATCCC125500.016.6596494
TAAATCC130050.016.6339043
CACGTTC119700.016.51515432
ACACGTT116250.016.47113631
TTCACAC116650.016.29670528
CCAAACG128750.016.13142614
AGTTCCG44650.015.5315295
GTTAAAT141100.015.5262841
GTCTAGT49350.015.5198181
CCCGCCT17200.014.6424117
Produced by FastQC (version 0.11.2)