FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_sq2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_sq2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11845199
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC62150.019.2075218
CCATGCC65500.018.5933199
TCACACG56150.017.5734229
AGTTCCG29700.016.961065
CACACGT63500.015.81026430
CAAACGG70600.015.19742715
GTCTAGT37750.014.8057611
TCCCATG83750.014.4598637
CGCGTGA27350.014.38082827
CGTACTG34400.014.3427454
GCGCGTG29250.014.15446926
ACACGTT71800.014.07871631
CCCGGGG7600.014.0728091
ATCGTAC36800.014.0634742
CACGTTC73050.013.9794932
TAAATCC83550.013.8754643
AAATCCC80350.013.8698364
ATGCGCG29300.013.77705724
CACGCCC17400.013.680943518
CCAAACG78500.013.66806214
Produced by FastQC (version 0.11.2)