FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_sq1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_sq1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17679650
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGC76300.017.9967848
TCACACG60900.017.84553729
CCATGCC84300.016.6569169
GTATATA51900.014.7573111
CACACGT75900.014.45454830
CGCGTGA35000.013.40620927
GCGCGTG35750.013.31797426
GTATTAG42800.013.2197041
TAGCCTA24350.013.03494610
TGCGCGT36850.013.01401125
CAAACGG91700.012.97967915
CACGCCC20250.012.94807718
ATGCGCG37400.012.91484324
TAAATCC102550.012.8855193
ATCCCAT117900.012.8467126
TCCCATG111950.012.7588457
CCGACCG17350.012.7264799
ACACGTT92950.012.1742931
GTATAAA74950.012.1521721
CACGTTC92300.012.07309932
Produced by FastQC (version 0.11.2)