FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_sq0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_sq0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22476152
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACACG94900.022.21350929
CCCATGC119500.021.7973588
CCATGCC129800.020.572559
GTATATA33800.019.1896861
CCGACCG33850.019.0592069
CACACGT111550.019.02120630
TAAATCC139300.017.684123
GTTAAAT148100.017.4250181
CCCGGGG11550.017.0261978
GTCTAGT68150.016.9592111
CACGCCC33650.016.81403518
TTCACAC127600.016.79095528
ATCCCCC23100.016.5777715
TAGCCTA34050.016.41405710
CACGTTC136050.016.20443332
CCGCGCG5550.016.1622669
CAAACGG141500.016.11610615
AGTTCCG57200.016.0444155
GTATAGA48700.015.8688611
AAATCCC146350.015.8185324
Produced by FastQC (version 0.11.2)