FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_other_stuff_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_other_stuff_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17208578
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAG94450.013.5891355
CTAATAC74550.013.5139863
GACGGAC84200.013.39834569
TCCGCAC36800.013.21844964
TAGGTAG32150.012.5561275
GTATATA135100.012.5132491
ACGGACT79550.012.49015469
GTATTAG64800.012.4586281
TAATACT132300.011.9441144
CCGCACC39350.011.92348965
CACGCAG53550.011.91870316
CGCGGCC19200.011.67969113
GTATAGA81850.011.6759021
GAGACGG137850.011.61252569
ATACAGG79200.011.5008746
ACTATGC84450.011.4799798
CGCACCT39700.011.47077166
TATATAC150500.011.3020883
TACAGGC55650.011.2836137
CTATGCA66750.011.2159629
Produced by FastQC (version 0.11.2)