FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_other_stuff_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_other_stuff_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12512441
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC24300.018.31433164
CCGCACC26100.017.31563465
CGCACCT26750.016.8948866
GTATTAG54600.016.4920461
GTATATA141900.015.3173921
TAGGTAG29750.014.9610625
CTAATAC60850.014.8557713
TATACTG82150.014.6160965
TATACAG89550.014.178885
ACTATGC62450.013.7565968
TAATACT105350.013.591524
GTATAGA61850.013.5548061
CATATAC83750.013.0183743
CCTAATA67200.012.6815322
CTATGCA52850.012.4034439
GTATAAT90600.012.1856441
ATTATAC95450.012.1817163
TATATAC162700.011.8755843
TTATACT86100.011.8615934
TATACGG43350.011.7796485
Produced by FastQC (version 0.11.2)