FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_hw4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_hw4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15308110
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC25150.017.96976541
ATGCCGT29600.015.2682344
CCCGGGG4300.015.2446331
CGGCCAG23250.014.6907531
TCACACG43950.014.67975429
CCCATGC52400.014.4854898
CCATGCC58300.014.025199
GACGCGC29800.013.89229738
CTCGCCG15000.013.80053928
CTTAAGG41300.013.7844613
CTAGTAG49750.013.31570153
GCCAGAC26100.013.2195113
CCTGCCG43400.012.79817716
ATCTAGT52700.012.7660281
CGATCCG7350.012.67355110
AGGCGAA81950.012.6717569
AGTTCCG27000.012.65127855
GGTCTCG29750.012.64012817
GTATGCC36600.012.63085742
TGACGCG33250.012.55459937
Produced by FastQC (version 0.11.2)