FastQCFastQC Report
Tue 3 Apr 2018
HLFF3BGX5_n01_hw3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFF3BGX5_n01_hw3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10854834
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC20500.022.55066941
CCCATGC65150.019.9644538
ATGCCGT23500.019.52505544
CCATGCC69000.019.3004179
TCACACG57100.018.97309129
CCCGGGG9100.018.9561941
CACACGT67500.016.202530
CAAACGG72850.016.19627215
AAATCCC75300.016.1739544
GTATGCC29200.015.83180542
GACGCGC12750.015.69373438
ATCCCAT85800.015.682526
GTTAAAT85750.015.6106181
AATCCCA83800.015.4392355
ACACGTT70950.015.36601331
TCCCATG86800.015.1042227
TAAATCC84350.015.0930073
TCTCGTA29000.014.98928938
CCAAACG81000.014.95004914
AGTTCCG27300.014.9127555
Produced by FastQC (version 0.11.2)